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dsm516 medium  (DSMZ)


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    DSMZ dsm516 medium
    Dsm516 Medium, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsm516+medium/Cupriavidus+necator/pmc10868218-19-23-7
    Average 95 stars, based on 215 article reviews
    dsm516 medium - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Modification:

    Article Title: Functional Analysis of the Glucan Degradation Locus in Caldicellulosiruptor bescii Reveals Essential Roles of Component Glycoside Hydrolases in Plant Biomass Deconstruction
    Article Snippet: .. For routine cultivation, a modified version of 574 DSM516 medium (DSMZ [http://www.dsmz.de]) was used, containing 0.33 g/L NH4Cl, 0.33 575 on O ctober 8, 2017 by S A M H O U S T O N S T A T E U N IV E R S IT Y http://aem .asm .org/ D ow nloaded from 25 g/L KCl, 0.33 g/L MgCl2 ● 6H2O, 0.14 g/L CaCl2 ● 2H2O, 0.16 mM sodium tungstate, 1 mL/L 576 trace element solution SL-10, 1 mL/L vitamin solution, 0.25 mg/L resazurin, 1 g/L L-Cysteine-577 HCl ● H20, 1 g/L sodium bicarbonate, 1 mM potassium phosphate, 5 g/L glucose or cellobiose. ..

    Article Title: Functional Analysis of the Glucan Degradation Locus in Caldicellulosiruptor bescii Reveals Essential Roles of Component Glycoside Hydrolases in Plant Biomass Deconstruction
    Article Snippet: .. For routine cultivation, a modified version of DSM516 medium (DSMZ) was used, containing 0.33 g/liter NH 4 Cl, 0.33 g/liter KCl, 0.33 g/liter MgCl 2 ·6H 2 O, 0.14 g/liter CaCl 2 ·2H 2 O, 0.16 mM sodium tungstate, 1 ml/liter trace element solution SL-10, 1 ml/liter vitamin solution, 0.25 mg/liter resazurin, 1 g/liter l -cysteine-HCl·H 2 O, 1 g/liter sodium bicarbonate, 1 mM potassium phosphate, and 5 g/liter glucose or cellobiose. ..

    Article Title: Engineering the cellulolytic extreme thermophile Caldicellulosiruptor bescii to reduce carboxylic acids to alcohols using plant biomass as the energy source.
    Article Snippet: .. Preparation of whole‐cell extracts and protein purification C. bescii strains DSMZ 6725 and MACB1038 were each grown in 1 L of modified DSM516 medium with glucose until the late-exponential phase. ..

    Article Title: Complete genome sequence for the extremely thermophilic bacterium Anaerocellum danielii (DSM:8977)
    Article Snippet: .. A. danielii culture was procured previously from DSMZ and maintained as −80°C freezer stocks and grown for genome sequencing at 75°C in modified DSM516 medium, as previously described ( ). .. Genomic DNA was extracted and purified from liquid cultures using the NEB Monarch Genomic DNA Purification Kit (New England Biolabs, USA) with the tissue lysis buffer and lysozyme.

    Protein Purification:

    Article Title: Engineering the cellulolytic extreme thermophile Caldicellulosiruptor bescii to reduce carboxylic acids to alcohols using plant biomass as the energy source.
    Article Snippet: .. Preparation of whole‐cell extracts and protein purification C. bescii strains DSMZ 6725 and MACB1038 were each grown in 1 L of modified DSM516 medium with glucose until the late-exponential phase. ..

    Sequencing:

    Article Title: Complete genome sequence for the extremely thermophilic bacterium Anaerocellum danielii (DSM:8977)
    Article Snippet: .. A. danielii culture was procured previously from DSMZ and maintained as −80°C freezer stocks and grown for genome sequencing at 75°C in modified DSM516 medium, as previously described ( ). .. Genomic DNA was extracted and purified from liquid cultures using the NEB Monarch Genomic DNA Purification Kit (New England Biolabs, USA) with the tissue lysis buffer and lysozyme.



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